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  • Lactate modulates zygotic genome activation through H3K18 lactylation rather than H3K27 acetylation.

Lactate modulates zygotic genome activation through H3K18 lactylation rather than H3K27 acetylation.

Cellular and molecular life sciences : CMLS (2024-07-12)
Yanhua Zhao, Meiting Zhang, Xingwei Huang, Jiqiang Liu, Yuchen Sun, Fan Zhang, Na Zhang, Lei Lei
ABSTRACT

In spite of its essential role in culture media, the precise influence of lactate on early mouse embryonic development remains elusive. Previous studies have implicated lactate accumulation in medium affecting histone acetylation. Recent research has underscored lactate-derived histone lactylation as a novel epigenetic modification in diverse cellular processes and diseases. Our investigation demonstrated that the absence of sodium lactate in the medium resulted in a pronounced 2-cell arrest at the late G2 phase in embryos. RNA-seq analysis revealed that the absence of sodium lactate significantly impaired the maternal-to-zygotic transition (MZT), particularly in zygotic gene activation (ZGA). Investigations were conducted employing Cut&Tag assays targeting the well-studied histone acetylation and lactylation sites, H3K18la and H3K27ac, respectively. The findings revealed a noticeable reduction in H3K18la modification under lactate deficiency, and this alteration showed a significant correlation with changes in gene expression. In contrast, H3K27ac exhibited minimal correlation. These results suggest that lactate may preferentially influence early embryonic development through H3K18la rather than H3K27ac modifications.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Acetyl-Histone H3 (Lys18) antibody, Rabbit monoclonal, recombinant, expressed in HEK 293 cells, clone RM166, purified immunoglobulin
Sigma-Aldrich
Anti-acetyl-Histone H3 (Lys9) Antibody, Upstate®, from rabbit