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product name
C2, 94101906
biological source
rat liver
growth mode
Adherent
karyotype
Hyperdiploid, 52 Chromosomes
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat hepatoma [HGPRT-]
Cell Line Description
Full name Faof1C2 is a dedifferentiated variant of Fao. These cells continue to express tyrosine aminotransferase, but the liver-specific markers are mostly absent.
Application
Gene expression analysis.
Culture Medium
Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split semi-confluent cultures 1:50 i.e. seeding at 2x1000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density is reached at 10x10,000,000,000 cells/10cm2 dish.
Other Notes
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Certificates of Analysis (COA)
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