41782

MRS Agar, Vegitone

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): DeMan, Rogosa and Sharpe Agar, Vegitone, Vegitone MRS Agar
• UNSPSC Code: 41171605
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15.0 g/L ; ammonium citrate, 2.0 g/L ; bromocresol green, 0.04 g/L ; captan, 0.004 g/L ; dextrose, 20.0 g/L ; dipotassium phosphate, 2.0 g/L ; magnesium sulfate, 0.1 g/L ; manganese sulfate, 0.05 g/L ; 2-phenylethyl alcohol, 3.0 g/L ; proteose peptone (vegetable), 10.0 g/L ; sodium acetate, 5.0 g/L ; yeast extract, 5.0 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 5.5±0.2 (25 °C)
• application(s): agriculture; bioburden testing; environmental; food and beverages; microbiology
• suitability: selective and differential for Lactobacillus spp.; selective by low pH for Bifidobacterium spp.

Description

General description

This MRS Agar is free of animal derived material. It is recommended for the isolation and cultivation of Lactobacillus species.

Application

MRS Agar, Vegitone has been used for culturing yogurt to analyze lactic acid bacteria (LAB). It has also been used in culturing of Lactobacillus reuteri colonies.

Preparation Note

Suspend 62.2 g in 1000 ml distilled water containing 1 ml polysorbate 80 (TWEEN^® 80; Cat. No. P8074). Boil to dissolve the medium completely and mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves