Yes, the Cell Signaling Lysis Buffer (43-040) can be used in the Mouse Cytokine Milliplex kit (MCYTOMAG-70K). The buffer was tested at dilutions of 1:2, 1:5, and 1:10 in Assay Buffer, with the 1:5 dilution showing comparable results to the Assay Buffer for most cytokines and the 1:10 dilution providing optimal results if the samples can tolerate that level of dilution.
MCYTMAG-70K-PX32
MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 32 Plex - Immunology Multiplex Assay
MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 32 Plex - Immunology Multiplex Assay
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Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
Synonym(s):
Luminex® Mouse cytokine immunoassay, Millipore Mouse cytokine immunoassay, Mouse cytokine Multiplex Assay
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
Pricing and availability is not currently available.
Recommended Products
Quality Level
species reactivity
mouse
manufacturer/tradename
Milliplex®
assay range
accuracy: 85-107%
standard curve range: 3.2-10,000 pg/mL
technique(s)
multiplexing: suitable
compatibility
configured for Premixed
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
General description
The immune system is an organized complex network of biological structures and processes that protect against disease. For example, cytokine and chemokines mediate interactions between cells directly, regulating target immune cell responses. These responses result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity. In addition, cytokine and chemokine research is essential in understanding the immune system and its multi-faceted response to most antigens, as well as disease states such as autoimmune disease, allergic reactions, sepsis, and cancer.
To identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
More flexible plate and plate washer options
MILLIPLEX® Mouse Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Panel Type: Cytokines/Chemokines
To identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
More flexible plate and plate washer options
- Improved performance with turbid serum/plasma samples
- Assay results equivalent to non- beads
- Automated washing avoids many problems associated with vacuum filtration washing
MILLIPLEX® Mouse Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Panel Type: Cytokines/Chemokines
Specificity
Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Application
- Analytes: Eotaxin/CCL11, G-CSF, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IP-10, KC, LIF, LIX, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-2, RANTES, TNF-α, VEGF
- Recommended Sample type: plasma, serum, or cell culture supernatant
- Recommended Sample dilution: 1:2 diluted plasma or serum
- Assay Run Time: Overnight or one day. For best results an overnight incubation is recommended.
- Research Category: Inflammation & Immunology
Packaging
96 well plate
Premixed beads for your convenience.
Storage and Stability
Recommended storage for kit components is 2 - 8°C.
Other Notes
Sensitivity: Refer to kit protocol for sensitivities for individual cytokines.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
Storage Class Code
10 - Combustible liquids
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Can the Cell Signaling Lysis Buffer (43-040) be used in the Mouse Cytokine Milliplex kit (MCYTOMAG-70K)?
1 answer-
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How do I enter the high, low, and expected concentrations for the kit QCs into Xponent? Since the QC range sheet only includes the high and low ranges, could you please provide the expected concentration as well?
1 answer-
A quality control range sheet is available, providing different values for quality controls. The expected average per range can be calculated by adding the high and low numbers for each marker, then dividing the total by 2.
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