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AB3046

Sigma-Aldrich

Anti-Uncoupling Protein 3 Antibody

Chemicon®, from rabbit

Synonym(s):

UCP3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_003356.2
NM_022803.1

UniProt accession no.

P55916

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... UCP3(7352)
rat ... Ucp3(25708)

General description

Human UCP3 long form (UCP3L) is a 312 aa mitochondrial uncoupling protein. It is only 57% and 73% homologous with human UCP1 and UCP2 respectively. Like other UCPs, UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and is only 275 aa long. UCP3 has preferential expression in muscle and it is unaffected by cold acclimation.

Specificity

Uncoupling Protein 3 (UCP3), human. It is expected that the antibody will recognize both the long and short forms (30-33 kDa & 24-27 kDa) however splice variants are expected and precise reactivity profile has yet to be determined. Human UCP3 long form (UCP3L) is a 312 aa mitochondrial uncoupling protein (Boss et al. 1997; Vidal-Puig et al. 1997). It is only 57% and 73% homologous with human UCP1 and UCP2 respectively. Like other UCPs, UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and is only 275 aa long (Vidal-Puig et al. 1997). UCP3 has preferential expression in muscle and it is unaffected by cold acclimation.

Immunogen

14 amino acid peptide sequence (designated UCP32) mapping near the C-terminus of human UCP3 (Boss et al. 1997). The UCP32 peptide has no significant homology with UCP1 or UCP2.

Application

Anti-Uncoupling Protein 3 Antibody is an antibody against Uncoupling Protein 3 for use in ELISA, IH & WB.
Research Category
Metabolism
Research Sub Category
Ion & Transport Channels
Western Blotting: 1-10 μg/mL using Chemiluminescence technique.

Immunohistochemistry: 2-20 μg/mL on formaldehyde fixed tissue.

ELISA: (1:10,000-1:100,000 using 50-100 ng UCP32 control peptide/well).

Optimal working dilutions must be determined by end user.

Target description

30-33 kDa & 24-27 kDa

Physical form

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
ImmunoAffinity Purified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Muscle tissue, Blocking peptide for UCP3 is available (Catalogue No. AG769).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xiaoyan Sun et al.
American journal of physiology. Endocrinology and metabolism, 285(3), E512-E520 (2003-05-02)
Uncoupling protein 3 (UCP3) is a member of the mitochondrial transporter superfamily that is expressed primarily in skeletal muscle. UCP3 is upregulated in various conditions characterized by skeletal muscle atrophy, including hyperthyroidism, fasting, denervation, diabetes, cancer, lipopolysaccharide (LPS), and treatment
Carine Duval et al.
FEBS letters, 581(5), 955-961 (2007-02-17)
Fatty acids induced an increase in reactive oxygen species (ROS) and enhanced NF-kappaB activation in L6 myotubes differentiated in culture. Palmitate proved more effective than oleate in eliciting these effects. The induction of uncoupling protein-3 (UCP3) at levels similar to
Maria Fernström et al.
The Journal of physiology, 554(Pt 3), 755-763 (2003-11-25)
Mitochondrial proteins such as uncoupling protein 3 (UCP3) and adenine nucleotide translocase (ANT) may mediate back-leakage of protons and serve as uncouplers of oxidative phosphorylation. We hypothesized that UCP3 and ANT increase after prolonged exercise and/or endurance training, resulting in
M Mogensen et al.
The Journal of physiology, 571(Pt 3), 669-681 (2006-01-21)
The purpose of this study was to investigate the hypothesis that cycling efficiency in vivo is related to mitochondrial efficiency measured in vitro and to investigate the effect of training status on these parameters. Nine endurance trained and nine untrained
Fenofibrate activates the biochemical pathways and the de novo expression of genes related to lipid handling and uncoupling protein-3 functions in liver of normal rats.
Elena Silvestri, Pieter de Lange, Maria Moreno, Assunta Lombardi, Maurizio Ragni et al.
Biochimica et Biophysica Acta null
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