345832

Galectin-3C, Human, Recombinant, E. coli

• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Assay: ≥95% (SDS-PAGE)
• Quality Level: 100
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• impurities: ≤1 EU/μg protein Endotoxin
• solubility: sterile PBS: 1 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Recombinant, human galectin-3 expressed in E. coli and subsequently cleaved with Clostridium histiolyticum collagenase type VII to yield galectin-3C. The resulting galectin-3C is purified by affinity chromatography. Galectin-3C is a truncated form of galectin-3 that contains the carbohydrate binding domain but lacks the 107 amino acids at the N-terminus of galectin-3. It is suggested to play a key role in autoimmune diseases, allergic reactions, inflammation, tumor cell metastasis, atherosclerosis, and diabetes.

Recombinant, human galectin-3 expressed in E. coli and subsequently cleaved with Clostridium histolyticum collagenase type VII to yield galectin-3C. The resulting galectin-3C is purified by affinity chromatography. Galectin-3C is a truncated form of galectin-3 that contains the carbohydrate binding domain but lacks the 107 amino acids at the N-terminus of galectin-3. It is suggested to play a key role in autoimmune diseases, allergic reactions, inflammation, tumor cell metastasis, atherosclerosis, and diabetes.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from deionized water, 2µg lactose/µg lectin.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions (in the presence of a molar excess of lactose) are stable for up to 4 months at -20°C. For use in carbohydrate binding assays the solution should be dialyzed to remove the lactose stabilizer.

Other Notes

Hoyer K.K., et al. 2004. Am. J. Pathol.164, 893.
John C.M., et al. 2003. Clin. Cancer Res.9, 2374.
Iurisci, I., et al. 2000. Clin Cancer Res6, 1389.
Van Den Brule, F. A., et al. 2000. Int. J. Cancer89, 361.
Inohara, H. 1999. Cancer85, 2475.
Nakamura, M., et al. 1999. Int. J. Oncol.15, 143.
Idikio, H., et al. 1998. Int. J. Oncol.12, 1287.
Ochieng, J., et al. 1998. Biochim. Biophys. Acta.1379 97.
Castronovo, V., et al. 1996. J. Pathol.179, 43.
Liu, F.T., et al. 1996. Biochemistry35 6073.
Schoeppner, H. L., et al. 1995. Cancer75, 2818.
Massa, S.M., et al. 1993. Biochemistry32, 260.
Oda, Y., et al. 1991. Gene99, 279.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable