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  • Ultrastructure of the hemocytes of Cetonischema aeruginosa larvae (Coleoptera, Scarabaeidae): involvement of both granulocytes and oenocytoids in in vivo phagocytosis.

Ultrastructure of the hemocytes of Cetonischema aeruginosa larvae (Coleoptera, Scarabaeidae): involvement of both granulocytes and oenocytoids in in vivo phagocytosis.

Tissue & cell (2003-08-19)
P G Giulianini, F Bertolo, S Battistella, G A Amirante
ABSTRACT

In the context of comparative studies on immunity defence mechanisms of adults and larvae of the coleopteran Cetonischema aeruginosa (Drury, 1770) the ultrastructure of the circulating hemocytes of the third instar larval stage has been investigated by means of light and transmission electron microscopy (TEM). Six types of hemocytes were found in the hemolymph of C. aeruginosa and they were identified as prohemocytes, granulocytes, plasmatocytes, coagulocytes, oenocytoids and spherule cells. In order to identify the "professional" phagocyte cell, phagocytosis assays were performed in vivo by injection of 0.9 microm carboxylate-modified polystyrene latex beads. It was demonstrated that the granulocytes and the oenocytoids of C. aeruginosa were the only hemocyte types involved in this cellular response.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Latex beads, carboxylate-modified polystyrene, fluorescent yellow-green, aqueous suspension, 1.0 μm mean particle size
Sigma-Aldrich
Latex beads, carboxylate-modified polystyrene, fluorescent yellow-green, aqueous suspension, 0.03 μm mean particle size
Sigma-Aldrich
Latex beads, carboxylate-modified polystyrene, fluorescent yellow-green, aqueous suspension, 2.0 μm mean particle size
Sigma-Aldrich
Latex beads, carboxylate-modified polystyrene, fluorescent red, aqueous suspension, 2.0 μm mean particle size
Sigma-Aldrich
Latex beads, carboxylate-modified polystyrene, fluorescent orange, aqueous suspension, 0.5 μm mean particle size
Sigma-Aldrich
Latex beads, carboxylate-modified, streptavidin-labeled, blue, buffered aqueous suspension, 0.25 μm mean particle size
Sigma-Aldrich
Latex beads, carboxylate-modified polystyrene, fluorescent red, aqueous suspension, 0.5 μm mean particle size