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  • Phagocytotic activities of latex beads by FRTL cell: possible interaction of cell surface glycoprotein.

Phagocytotic activities of latex beads by FRTL cell: possible interaction of cell surface glycoprotein.

Acta endocrinologica (1988-02-01)
K Takazawa, T Endo, T Onaya
ABSTRACT

We investigated the phagocytic activity of cultured rat thyroid cells (FRTL) by using latex beads. FRTL cells ingested many carboxylate-modified polystyrene latex beads (c-latex beads), which had carboxyl residues on the surface, whereas they ingested few polystyrene latex beads (p-latex beads), which had no electrostatic charge. Phagocytotic activity of c-latex beads was stimulated by TSH in a dose-related manner. Dibutyryl, 8-bromo-cAMP, and forskolin also stimulated this process. Tunicamycin significantly inhibited dibutyryl cAMP-stimulated phagocytotic activity of c-latex beads by FRTL cells. N-acetylglucosaminidase also inhibited dibutyryl cAMP-stimulated phagocytosis. These results suggest that the interaction between negative surface charge of c-latex beads and positive surface charge probably induced by N-acetylglucosamine in the cell surface glycoprotein, might play an important role in the phagocytosis of FRTL cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Latex beads, carboxylate-modified polystyrene, fluorescent yellow-green, aqueous suspension, 0.03 μm mean particle size
Sigma-Aldrich
Latex beads, carboxylate-modified polystyrene, fluorescent yellow-green, aqueous suspension, 1.0 μm mean particle size
Sigma-Aldrich
Latex beads, carboxylate-modified polystyrene, fluorescent yellow-green, aqueous suspension, 2.0 μm mean particle size
Sigma-Aldrich
Latex beads, carboxylate-modified polystyrene, fluorescent red, aqueous suspension, 2.0 μm mean particle size
Sigma-Aldrich
Latex beads, carboxylate-modified polystyrene, fluorescent orange, aqueous suspension, 0.5 μm mean particle size
Sigma-Aldrich
Latex beads, carboxylate-modified, streptavidin-labeled, blue, buffered aqueous suspension, 0.25 μm mean particle size
Sigma-Aldrich
Latex beads, carboxylate-modified polystyrene, fluorescent red, aqueous suspension, 0.5 μm mean particle size