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Key Documents

G005539

HPLC Analysis of Fusarium Mycotoxins on Ascentis® Express Phenyl-Hexyl

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)
mobile phase: [A] water, acetonitrile, 90:10; [B] acetonitrile
gradient: 0% B held for 0.5 min; to 15% B in 1 min; held at 15% B for 6 min
flow rate: 0.3 mL/min
pressure: 192 bar (2780 psi)
column temp.: 30 °C
detector: UV, 218 nm
injection: 10 μL
sample: 5 mg/L ea. in 95:5, water:acetonitrile

suitability

application for HPLC

application(s)

agriculture
food and beverages

Analysis Note

The trichothecene vomitoxins of Fusarium are significant plant pathogens of grain and forage crops that can lead to anorexic effects with livestock. This application demonstrates resolution of deoxynivalenol and three of its analogs.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Nivalenol hydrate
    15-Acetyldeoxynivalenol solution
    3-Acetyldeoxynivalenol

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