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N2752

Sigma-Aldrich

4-Nitrophenyl palmitate

lipase substrate, chromogenic, ≥98% (TLC), powder

Synonym(s):

p-Nitrophenyl palmitate, Hexadecanoic acid 4-nitrophenyl ester

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About This Item

Empirical Formula (Hill Notation):
C22H35NO4
CAS Number:
Molecular Weight:
377.52
Beilstein:
1891754
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83

product name

4-Nitrophenyl palmitate, lipase substrate

Assay

≥98% (TLC)

form

powder

solubility

chloroform: 100 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

CCCCCCCCCCCCCCCC(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C22H35NO4/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-22(24)27-21-18-16-20(17-19-21)23(25)26/h16-19H,2-15H2,1H3

InChI key

LVZSQWIWCANHPF-UHFFFAOYSA-N

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General description

4-Nitrophenyl palmitate is a substrate for lipase enzyme activity. Lipase hydrolyzes 4-nitrophenyl palmitate and yields the yellow colored product 4-nitrophenol, which is measurable spectrophotometrically at 410 nm. This method is advantageous due to its short reaction time and facile spectrophotometric analyses. The cell-bound lipase has preference for 4-nitrophenyl palmitate as substrate than the extracellular lipase.

Application

4-nitrophenyl palmitate has been used as a substrate for lipase enzyme activity.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Differences in hydrolytic abilities of two crude lipases from Geotrichum candidum 4013
Brabcova J, et al.
Yeast, 27(12), 1029-1038 (2010)
Microbial enzymatic activities in aerobic activated sludge model reactors
Li Y and Chrost RJ
Enzyme and Microbial Technology, 39(4), 568-572 (2006)
Khadijeh Mozaffari et al.
Molecules (Basel, Switzerland), 26(12) (2021-07-03)
Cannabidiol (CBD) is a hydrophobic non-psychoactive compound with therapeutic characteristics. Animal and human studies have shown its poor oral bioavailability in vivo, and the impact of consuming lipid-soluble CBD with and without food on gut bioaccessibility has not been explored.
Lipase and biosurfactant from Ochrobactrum intermedium strain MZV101 isolated by washing powder for detergent application
Zarinviarsagh M, et al.
Lipids in Health and Disease, 16(1), 177-177 (2017)
Kai Yuan et al.
Journal of industrial microbiology & biotechnology, 46(8), 1091-1101 (2019-06-21)
Metabolic fluxes during lipase production by Bacillus subtilis CICC 20034 in synthetic medium were studied using metabolic flux analysis (MFA). The MFA showed that lipase production was dependent on, and coupled to the tributyrin uptake rate, formation of biomass, lactate

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