This product can be dissolved in a given target cell culture medium, e.g., non-complex media (MEM, RPMI-1640), to a 1X solution.
I3146
ITS Liquid Media Supplement (100×)
liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
Human ITS Liquid Media, Insulin-transferrin-sodium selenite
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About This Item
UNSPSC Code:
12352205
NACRES:
NA.75
Pricing and availability is not currently available.
Recommended Products
sterility
sterile-filtered
Quality Level
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
impurities
endotoxin, tested
shipped in
ambient
storage temp.
2-8°C
Application
Biochem/physiol Actions
Components
Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.
Other Notes
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.
Preparation Note
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.
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What should you use to dilute this to a 1X solution?
1 answer-
Helpful?
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What are the product numbers for the stand alone components in I3146?
1 answer-
The product numbers for the stand-alone components in I3146 are as follows:
Insulin – I2643 or I3536 (sterile)
Apo-Transferrin – T2252
Sodium Selenite – S5261
EBBS – E3024Helpful?
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The ITS product #I3146 has been on back order for a while. Is there an alternative with similar components?
1 answer-
Based on the Media Supplement Formulation, the closest alternative to product No. I3146 is the I2521 ITS+1 Liquid Media Supplement (100×), which also contains BSA and Linoleic acid.
Helpful?
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