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Key Documents

811-500

Sigma-Aldrich

Human Preadipocyte Growth Medium (500ml)

Synonym(s):

Primary Cell Culture Media

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

liquid

Quality Level

100

shelf life

6 mo.

manufacturer/tradename

Cell Applications, Inc

shipped in

wet ice

storage temp.

2-8°C

General description

All-in-one ready-to-use media

Application

Human Preadipocyte Growth Medium (500ml) has been used in culturing of human preadipocytes.

Quality

Each lot was tested for the ability to support plating, spreading and proliferation

Preparation Note

contained in protocol

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Infiltration of immune cells into adipose tissue is associated with chronic low-grade inflammation in obese individuals. To better understand the crosstalk between immune cells and adipocytes, in vivo-like in vitro models are required. Conventionally transwell culture plates are used for
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Triiodothyronine (T3) and irisin (I) can modulate metabolic status, increase heat production, and promote differentiation of white adipose tissue (WAT) into brown adipose tissue (BAT). Herein, human subcutaneous white adipocytes were treated with 10 nM T3 or 20 nM I for 24 h

Protocols

Human Preadipocytes (HPAd) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Preadipocytes (HPAd) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Preadipocytes (HPAd) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Preadipocytes (HPAd) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

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