ABN302
Anti-Complex I-75kD Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial, Complex I-75kD, CI-75kD
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
species reactivity (predicted by homology)
bovine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NDUFS1(4719)
General description
Complex I-75kD (CI-75kD) is also called NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (NDUFS1). Complex I-75kD is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which transfers electrons from NADH to the respiratory chain, and is thought to be part of the minimal assembly required for catalysis. Complex I-75kD defects are thought to be a cause of Mitochondrial complex I deficiency (MT-C1D).
Immunogen
KLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Complex I-75kD.
Application
Anti-Complex I-75kD, Cat. No. ABN302, is a rabbit polyclonal antibody that detects Complex I-75kD and is tested for use in Western Blotting and Immunohistochemistry.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
Tested Applications
Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected Complex I-75kD in rat brain, human brain, and human heart tissue lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Complex I-75kD in human kidney and human heart tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected Complex I-75kD in rat brain, human brain, and human heart tissue lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Complex I-75kD in human kidney and human heart tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Quality
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Complex I-75kD in mouse brain tissue lysates.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Complex I-75kD in mouse brain tissue lysates.
Target description
~75 kDa observed
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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