69066-M

Recombinant Enterokinase

• Synonym(s): Enterokinase Enzyme, Enterokinase Protein, Recombinant Enterokinase
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• biological source: bovine
• Quality Level: 200
• recombinant: expressed in E. coli
• form: liquid
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• technique(s): targeted protein degradation: suitable
• suitability: suitable for enzyme test
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C

Description

General description

Recombinant Enterokinase (rEK) is a highly purified preparation of the catalytic subunit of bovine enterokinase, a serine protease which recognizes the identical cleavage site as the native enzyme (AspAspAspAspLys↓) and has similar enzymatic activity. This enzyme is produced in E. coli and purified to yield the highest activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a solution in 50% glycerol. EC 3.4.21.9, M.W. 26,300.

Components

•50 URecombinant Enterokinase

•2 ml1X rEK Dilution/Storage Buffer

•1 ml10X rEK Cleavage Buffer

•10 µgCleavage Control Protein

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Unit Definition

One unit is defined as the amount of enzyme that will cleave 50 µg of fusion protein in 16 hours at 23°C, in a buffer containing 20 mM Tris-HCl pH 7.4, 50 mM NaCl, 2 mM CaCl₂.

Other Notes

Collins-Racie, L.A. et al. 1995. Bio/Technology 13, 982.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable