HPLC Analysis of Antihypertensive and Diuretic Drugs on Discovery^® RP-Amide C16

column

Discovery RP-AmideC16, 15 cm × 4.6 mm I.D., 5 μm particles (505013)

mobile phase

[A] methanol; [B] 25 mM potassium phosphate, dibasic, pH 7.0 (22:78, A:B)

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 254 nm

injection

10 μL