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  • Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy.

Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy.

PloS one (2017-01-21)
Dieanira Erudaitius, Andrew Huang, Sarah Kazmi, Garry R Buettner, Victor G J Rodgers
ABSTRACT

Cancer cell toxicity to therapeutic H2O2 varies widely depending on cell type. Interestingly, it has been observed that different cancer cell types have varying peroxiporin expression. We hypothesize that variation in peroxiporin expression can alter cell susceptibility to therapeutic H2O2 concentrations. Here, we silence peroxiporin aquaporin-3 (AQP3) on the pancreatic cancer cell line MIA PaCa-2 and compare clonogenic survival response to the wild-type. The results showed a significantly higher surviving fraction in the clonogenic response for siAQP3 MIA PaCa-2 cells at therapeutic H2O2 doses (P < 0.05). These results suggest that peroxiporin expression is significant in modulating the susceptibility of cancer cells to ascorbate therapy.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
MISSION® esiRNA, targeting human AQP3
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Monoclonal Anti-AQP8 antibody produced in mouse, clone 1F8, purified immunoglobulin, buffered aqueous solution
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Bovine Serum Albumin, heat shock fraction, pH 7, ≥98%
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Anti-AQP1 antibody produced in rabbit, 1 mg/mL, affinity isolated antibody
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Anti-AQP3 antibody produced in rabbit, 1 mg/mL, affinity isolated antibody
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Accutase® solution, sterile-filtered, suitable for cell culture