Skip to Content
Merck
  • Epidermal growth factor receptor-targeted immunoliposomes for delivery of celecoxib to cancer cells.

Epidermal growth factor receptor-targeted immunoliposomes for delivery of celecoxib to cancer cells.

International journal of pharmaceutics (2015-01-18)
Yanuar Dwi Putra Limasale, Ayşen Tezcaner, Can Özen, Dilek Keskin, Sreeparna Banerjee
ABSTRACT

Cyclooxygenase-2 (COX-2) is highly expressed in many different cancers. Therefore, the inhibition of the COX-2 pathway by a selective COX-2 inhibitor, celecoxib (CLX), may be an alternative strategy for cancer prevention and therapy. Liposomal drug delivery systems can be used to increase the therapeutic efficacy of CLX while minimizing its side effects. Previous studies have reported the encapsulation of CLX within the non-targeted long circulating liposomes and functional effect of these formulations against colorectal cancer cell lines. However, the selectivity and internalization of CLX-loaded liposomes can further be improved by grafting targeting ligands on their surface. Cetuximab (anti-epidermal growth factor receptor - EGFR - monoclonal antibody) is a promising targeting ligand since EGFR is highly expressed in a wide range of solid tumors. The aim of this study was to develop EGFR-targeted immunoliposomes for enhancing the delivery of CLX to cancer cells and to evaluate the functional effects of these liposomes in cancer cell lines. EGFR-targeted ILs, having an average size of 120nm, could encapsulate 40% of the CLX, while providing a sustained drug release profile. Cell association studies have also shown that the immunoliposome uptake was higher in EGFR-overexpressing cells compared to the non-targeted liposomes. In addition, the CLX-loaded-anti-EGFR immunoliposomes were significantly more toxic compared to the non-targeted ones in cancer cells with EGFR-overexpression but not in the cells with low EGFR expression, regardless of their COX-2 expression status. Thus, selective targeting of CLX with anti-EGFR immunoliposomes appears to be a promising strategy for therapy of tumors that overexpress EGFR.

MATERIALS
Product Number
Brand
Product Description

Supelco
Rhodamine B, analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, NMR reference standard
Pricing and availability is not currently available.
Supelco
Cholesterol solution, certified reference material, 10 mg/mL in chloroform
Pricing and availability is not currently available.
Sigma-Aldrich
SyntheChol® NS0 Supplement, 500 ×, synthetic cholesterol, animal component-free, aqueous solution, sterile-filtered, suitable for cell culture
Pricing and availability is not currently available.
Supelco
Chloroform, analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
Pricing and availability is not currently available.
Sigma-Aldrich
Rhodamine B, for fluorescence
Pricing and availability is not currently available.
Sigma-Aldrich
Cholesterol, tested according to Ph. Eur.
Pricing and availability is not currently available.
Supelco
Methanol, analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
Rhodamine B, ≥95% (HPLC)
Pricing and availability is not currently available.
Sigma-Aldrich
Cholesterol, Sigma Grade, ≥99%
Pricing and availability is not currently available.
Sigma-Aldrich
Cholesterol, powder, BioReagent, suitable for cell culture, ≥99%
Pricing and availability is not currently available.
Sigma-Aldrich
1,2-Distearoyl-sn-glycero-3-phosphocholine, ≥99%
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer
Pricing and availability is not currently available.
Sigma-Aldrich
Cholesterol, from sheep wool, ≥92.5% (GC), powder
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Pricing and availability is not currently available.
Supelco
Cholesterol, Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, Absolute - Acetone free
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, ACS spectrophotometric grade, ≥99.9%
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, Laboratory Reagent, ≥99.6%
Pricing and availability is not currently available.
Supelco
Methanol, Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.
Supelco
Chloroform, Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, ACS spectrophotometric grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 0.5-1.0% ethanol as stabilizer
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer
Pricing and availability is not currently available.
SAFC
Cholesterol, from sheep wool, Controlled origin, meets USP/NF testing specifications
Pricing and availability is not currently available.
Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, 98%
Pricing and availability is not currently available.
Supelco
Rhodamine B solution, 0.2% in isopropanol, for TLC derivatization
Pricing and availability is not currently available.