- High-performance liquid chromatographic separation and on-line mass spectrometric detection of saturated and unsaturated oligogalacturonic acids.
High-performance liquid chromatographic separation and on-line mass spectrometric detection of saturated and unsaturated oligogalacturonic acids.
Carbohydrate research (2002-12-21)
Thomas Stoll, Andreas Schieber, Reinhold Carle
PMID12493233
ABSTRACT
A method for the simultaneous determination of saturated and unsaturated oligogalacturonic acids up to degree of polymerization (dp) of 7 by high-performance liquid chromatography (HPLC) is presented. For this purpose, a Cyclobond I 2000 column and a volatile mobile phase consisting of ammonium formate and methanol were used, allowing direct coupling of HPLC to a mass spectrometer via an electrospray interface (ESI-MS) without additional desalting. The analytical system was used for the characterization of digests obtained by incubation of polygalacturonic acid with commercial enzyme preparations.
MATERIALS
Product Number
Brand
Product Description
Supelco
Astec® CYCLOBOND I 2000 Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
Astec® CYCLOBOND I 2000 Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
Supelco
Astec® CYCLOBOND I 2000 Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
Supelco
Astec® CYCLOBOND I 2000 Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm