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  • Novel bacterial molybdenum and tungsten enzymes: three-dimensional structure, spectroscopy, and reaction mechanism.

Novel bacterial molybdenum and tungsten enzymes: three-dimensional structure, spectroscopy, and reaction mechanism.

Biological chemistry (2005-10-13)
Matthias Boll, Bernhard Schink, Albrecht Messerschmidt, Peter M H Kroneck
ABSTRACT

The molybdenum enzymes 4-hydroxybenzoyl-CoA reductase and pyrogallol-phloroglucinol transhydroxylase and the tungsten enzyme acetylene hydratase catalyze reductive dehydroxylation reactions, i.e., transhydroxylation between phenolic residues and the addition of water to a triple bond. Such activities are unusual for this class of enzymes, which carry either a mononuclear Mo or W center. Crystallization and subsequent structural analysis by high-resolution X-ray crystallography has helped to resolve the reaction centers of these enzymes to a degree that allows us to understand the interaction of the enzyme and the respective substrate(s) in detail, and to develop a concept for the respective reaction mechanism, at least in two cases.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Tungsten, foil, thickness 0.25 mm, ≥99.9% trace metals basis
Tungsten, wire, straight, 100mm, diameter 0.25mm, 99.9+%
Tungsten, foil, 0.1m coil, thickness 0.076mm, coil width 2mm, as rolled, 99.95%
Tungsten, foil, 0.2m coil, thickness 0.05mm, coil width .51mm, as rolled, 99.95%
Tungsten, foil, 0.2m coil, thickness 0.6mm, coil width 1mm, as rolled, 99.95%
Tungsten, foil, 150x150mm, thickness 0.15mm, as rolled, 99.95%
Tungsten, foil, 50x50mm, thickness 0.125mm, as rolled, 99.95%
Tungsten, foil, 100x100mm, thickness 0.2mm, as rolled, 99.95%
Tungsten, foil, 0.2m coil, thickness 0.05mm, coil width 1.5mm, as rolled, 99.95%
Tungsten, foil, 10mm disks, thickness 1.0mm, as rolled, 99.95%
Tungsten, foil, 2m coil, thickness 0.035mm, coil width 1.5mm, as rolled, 99.95%
Tungsten, foil, 0.2m coil, thickness 0.02mm, coil width .1mm, as rolled, 99.95%
Tungsten, foil, 2m coil, thickness 0.6mm, coil width 1mm, as rolled, 99.95%
Tungsten, foil, 1m coil, thickness 0.6mm, coil width 1mm, as rolled, 99.95%
Tungsten, foil, 100x100mm, thickness 0.5mm, as rolled, 99.95%
Tungsten, foil, 2m coil, thickness 0.04mm, coil width 0.7mm, as rolled, 99.95%
Tungsten, foil, 50x50mm, thickness 0.2mm, as rolled, 99.95%
Tungsten, foil, 25mm disks, thickness 1.0mm, as rolled, 99.95%
Tungsten, foil, 2m coil, thickness 0.05mm, coil width .51mm, as rolled, 99.95%
Tungsten, foil, 2m coil, thickness 0.016mm, coil width .36mm, as rolled, 99.95%
Tungsten, foil, 1m coil, thickness 0.025mm, coil width 1mm, as rolled, 99.95%
Tungsten, foil, 2m coil, thickness 0.05mm, coil width 1.5mm, as rolled, 99.95%
Tungsten, foil, 0.5m coil, thickness 0.08mm, coil width .9mm, as rolled, 99.95%
Tungsten, foil, 0.2m coil, thickness 0.10mm, coil width .9mm, as rolled, 99.95%
Tungsten, foil, 0.5m coil, thickness 0.6mm, coil width 1mm, as rolled, 99.95%
Tungsten, foil, 150x150mm, thickness 0.5mm, as rolled, 99.95%
Tungsten, foil, 50x50mm, thickness 0.3mm, as rolled, 99.95%
Tungsten, foil, 100x100mm, thickness 0.1mm, as rolled, 99.95%
Tungsten, foil, 50x50mm, thickness 0.5mm, as rolled, 99.95%
Tungsten, foil, 0.2m coil, thickness 0.035mm, coil width .5mm, as rolled, 99.95%