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  • The relative contribution of the small and large intestine to the absorption and metabolism of rutin in man.

The relative contribution of the small and large intestine to the absorption and metabolism of rutin in man.

Free radical research (2006-10-04)
Indu B Jaganath, William Mullen, Christine A Edwards, Alan Crozier
ABSTRACT

Tomato juice containing rutin (quercetin-3-rutinoside) was ingested by healthy volunteers and ileostomists. Blood and urine collected over 24 h were analysed by HPLC with photodiode array (PDA) and tandem mass spectrometric detection. Low concentrations of isorhamnetin-3-glucuronide (Cmax = 4.3 +/- 1.5 nmoles/l) and quercetin-3-glucuronide (Cmax = 12 +/- 2 nmoles/l) were detected in plasma of healthy subjects. Metabolites appeared in blood after 4 h indicating absorption from the large intestine. Nine metabolites of rutin were detected in urine but with considerable variation in total amount (40 +/- 1-4981 +/- 115 nmoles over 24 h). No metabolites were detected in plasma or urine of ileostomists and 86 +/- 3% of the ingested rutin was recovered in ileal fluid. In subjects with an intact large intestine, but not ileostomists, rutin was catabolised with the appearance of 3,4-dihydroxyphenylacetic acid, 3-methoxy-4-hydroxyphenylacetic acid and 3-hydroxyphenylacetic acid in urine accounting for 22% of rutin intake.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
3,4-Dihydroxyphenylacetic acid, 98%
Sigma-Aldrich
3-Hydroxyphenylacetic acid, ≥99%
Sigma-Aldrich
Homovanillic acid, Fluorimetric reagent