- High-performance liquid chromatographic determination of tocopherols and tocotrienols and its application to diets and plasma of Finnish men. I. Analytical method.
High-performance liquid chromatographic determination of tocopherols and tocotrienols and its application to diets and plasma of Finnish men. I. Analytical method.
International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition (1984-01-01)
V Piironen, P Varo, E L Syväoja, K Salminen, P Koivistoinen
PMID6735614
ABSTRACT
A HPLC Method is described for the determination of tocopherols and tocotrienols in human diets and plasma. After a room-temperature saponification diet samples were extracted with n-hexane. A direct hexane extraction was used for plasma samples. Using a normal-phase column at elevated temperature and a fluorescence detector complete separation of all four tocopherols, alpha-, beta-, gamma-tocotrienols and BHA and good reproducibility and sensitivity were obtained. The recovery of tocopherols added to diet samples was 99% for alpha-tocopherol, 95% for beta-tocopherol, 99% for gamma-tocopherol and 80% for delta-tocopherol. The recovery of alpha-tocopherol added into plasma was 99%.
MATERIALS