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G005601

HPLC Analysis of Levothyroxine and Liothyronine on Ascentis® Express ES-Cyano

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express ES-Cyano, 10 cm x 2.1 mm I.D., 2.7 μm particles (53491-U)
mobile phase: [A] water with 0.05% phosphoric acid; [B] acetonitrile with 0.05% phophoric acid; (60:40, A:B)
flow rate: 1.5 mL/min
pressure: 3270 psi (225 bar)
column temp.: 30 °C
detector: UV, 225 nm
injection: 5 μL
sample: 20 μg/mL in mobile phase

suitability

application for HPLC

application(s)

clinical

Analysis Note

This application demonstrates the suitabilty of Ascentis Express ES-Cyano for the analysis of Levothyroxine according to USP Monograph 3301 with Liothyronine as a reference standard.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    3,3′,5-Triiodo-L-thyronine sodium salt, powder, BioReagent, suitable for cell culture
    Levothyroxine sodium hydrate, PESTANAL®, analytical standard

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