SAB4200810

Anti-Podocin, Mouse monoclonal

clone PD-65, purified from hybridoma cell culture

• Synonym(s): NPHS2
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: PD-65, monoclonal
• form: buffered aqueous solution
• mol wt: ~42 kDa
• species reactivity: bovine, rat, human, mouse
• packaging: antibody small pack of 25 μL
• concentration: 1 mg/mL
• technique(s): immunoblotting: 0.25-0.5 μg/mL using using human HEK-293T cells.; immunofluorescence: 1-2 μg/mL using human HEK-293T cells.
• isotype: IgG2a
• UniProt accession no.: Q9NP85
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NPHS2(7827)

Description

General description

Podocytes are highly specialized visceral epithelial cells that cover the outer aspect of the glomerulus in the kidney. The foot processes of adjacent podocytes regularly interdigitate leaving filtration slits that are bridged by a thin slit diaphragm. This is an extracellular adherens-like junction that serves as a barrier to macromolecules and plays a crucial role in the glomerular filtration process. Podocin (NPHS2 protein) is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band-7-stomatin family of lipid raft-associated proteins. It is a hairpin-like integral membrane protein with intracellular N- and C- termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It interacts there with nephrin (NPHS1 protein), a protein critical in development and function of the kidney filtration barrier and the adapter protein CD2-AP. Podocin is anchored in the glycosphingolipids and cholesterol rich lipid rafts of the outer leaflet of the plasma membrane and is partially localized with the actin cytoskeleton. In vitro studies suggest its involvement in nephrin signaling facilitation via AP-1 in HEK cells. In addition to its presence in the kidney, it was also reported in rat nervous tissue and possibly in vascular smooth muscle.

Immunogen

Synthetic peptide of human Podocin , conjugated to KLH

Application

The antibody may be used in various immunochemical techniques including Immunobloting (~42kDa) and Immunofluorescence. Detection of the Podocin band by Immunoblotting is specifically inhibited by the immunogen.a

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable