GE27-0361-01
Ready-to-go™ T4 DNA Ligase
Cytiva 27-0361-01, sufficient for 50 reactions
Synonym(s):
T4 DNA ligase enzyme
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.53
Recommended Products
usage
sufficient for 50 reactions
manufacturer/tradename
Cytiva 27-0361-01
General description
Ready-To-Go™ T4 DNA Ligase. Catalyzes the formation of a phosphodiester bond between the 5′-phosphoryl group and the 3′-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction. Ligation of blunt-ended DNA is greatly stimulated by polyethylene glycol.
Application
Applications include self-circularization of linear DNA prior to transformation, ligation of insert to vector, and ligation of synthetic linkers to blunt-ended DNA. Each predispensed, single-reaction, ambient-temperature-stable tube contains a glassified reaction mix which, when reconstituted to a final reaction volume of 20 μL, yields a solution of reaction buffer, T4 DNA ligase, and ATP.
Features and Benefits
- For covalently-linking DNA fragments in as little as 30 min.
- Single-dose, ambient-temperature-stable reaction mixes.
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Ready-To-Go is a trademark of Cytiva
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Storage Class Code
13 - Non Combustible Solids
Certificates of Analysis (COA)
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