A member of the Endophilin family, Endophilin A1 is a protein containing SH3 domains, and has been implicated in the trafficking of clathrin-mediated synaptic vesicle endocytosis within the membrane. It was originally characterized as a presynaptically enriched protein that binds to endocytic proteins polyphosphoinositide synaptojanin and dynamin. Endophilin A1 has been implicated in endocytic vesicle budding when its function in cell-free systems and in living synapses has been disturbed. Along with the SH3 domain, Endophilin A1 contains a BAR domain in the N-terminal region, that has been found to induce and bind membrane curvature. This protein’s expression is localized to the brain, most specifically in the frontal cortex. Markedly high expression is observed in fetal cerebellum.
Specificity
Human (94% sequence homology).
The antibody recognizes Endophilin-A1.
Immunogen
KLH-conjugated linear peptide corresponding to mouse Endophilin-A1.
Application
This Anti-Endophilin-A1 Antibody is validated for use in WB for the detection of Endophilin-A1.
Western Blot Analysis: 1:500 dilution from a previous lot detected Endophilin-A1 in 10 µg of rat brain and mouse brain membrane tissue lysates.
Quality
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 1:500 dilution of this antibody detected Endophilin-A1 in 10 µg of mouse brain tissue lysate.
Target description
~ 42 kDa
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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