HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #2)
Materials
analytical column
Product No.
Description
Pricing
CONDITIONS
column
Discovery BIO Wide Pore C18, 15 cm × 4.6 mm I.D., 5 μm particles (568222-U)
mobile phase
[A] (80:20), 0.1%TFA in water:0.1% TFA in acetonitrile; [B] (66:34), 0.1% TFA in water:0.1% TFA in acetonitrile
flow rate
1 mL/min
column temp.
30 °C
detector
UV, 215 nm
injection
5 μL Sigma Peptide Mix P2693 (~25 μg ea. Peptide) (~25 μg ea. peptide)
sample
Sigma Peptide Mix (Cat. No. P2693)
Description
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
