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LC/MS (TOF) Analysis of 2C Amine Drugs of Abuse on Ascentis® Express HILIC

LC/MS (TOF) Analysis of 2C Amine Drugs of Abuse on Ascentis® Express HILIC application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express HILIC, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

mobile phase component

Product No.
Description
Pricing

Ammonium formate

eluent additive for LC-MS, LiChropur, ≥99.0%

standard

Product No.
Description
Pricing

4-Bromo-2,5-dimethoxyphenethylamine hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

2C-B-FLY hydrochloride solution

1 mg/mL in acetonitrile: water (9:1), ampule of 1 mL, certified reference material, Cerilliant®

2,5-Dimethoxy-4-(n)-propylthiophenethylamine hydrochloride solution

1.0 mg/mL in acetonitrile: water (9:1) (as free base), ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express HILIC, 5 cm x 2.1 mm I.D., 2.7 μm particles (53934-U)

mobile phase

2 mM ammonium formate in 90:10 (v:v) acetonitrile:water

flow rate

0.4 mL/min

pressure

943 psi (65 bar)

column temp.

35 °C

detector

TOF/MS

injection

2 μL

sample

300 ng/mL in acetonitrile

Description

Analysis Note

The 2C amines are a family of illicit, psychedelic phenethylamines. The term "2C" refers to the two carbon atoms between the benzene ring and the amino group in the parent molecular structure. A rapid separation of four 2C amine compounds is shown here on an Ascentis Express HILIC column with TOF-MS detection. Cerilliant CRMs were used to ensure reliable MS identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany