HPLC Analysis of Vitamins, Fat Soluble, on Ascentis^® Si

column

Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)

mobile phase

[A] hexane; [B] ethyl acetate

gradient

10 to 30% B in 10 min; held for 2 min; to 10% B in 1 min

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 290 nm

injection

10 μL

sample

as indicated in 96:4 (hexane:isopropanol)