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  • Analytical, nutritional and biological evaluation of various brands of fortified and non-fortified wheat flour.

Analytical, nutritional and biological evaluation of various brands of fortified and non-fortified wheat flour.

Pakistan journal of pharmaceutical sciences (2014-05-09)
Sohail Amjad, Khalid Hussain, Nadeem Irfan Bukhari, Muhammad Tanveer Khan, Abida Latif, Muhammad Islam, Sabiha Karim, Furqan Khurshid Hashmi, Amjad Hussain, Muhammad Zeeshan Danish
ABSTRACT

In Pakistan, a funded flour fortification program was launched for malnourished population, residing mainly in rural low income areas, but the urban population having comparatively better nutritional as well as economic status was focused wherein excessive intake of fortificants might cause complications. Therefore, the present study describes the physicochemical properties, elemental composition, nutritional components and hemoglobin/ferritin increasing potential of fortified and non-fortified flour. Domesticated chicken (Gallus gallus domesticus), either sex, age one month, weight 380 ± 18.28 g, were randomly segregated into 4 groups (n=6). The group I, II and III were fed on fortified flour, whereas group IV was fed on non-fortified flour for 30 days. The birds were weighed and blood samples of each of the birds were analyzed for determination of markers of iron status, hemoglobin (Hb) and serum ferritin (SF). Moisture, ash and iron contents were found to be lower in non-fortified flour than that of the fortified samples. Hb and SF levels in groups fed on fortified flour were significantly higher than the one received non-fortified flour (P < 0.05). The consumption of iron-fortified flour increases iron stores in the body without any further complication but long-term usage needs to be monitored.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)
Iron, IRMM®, certified reference material, 0.5 mm wire
Sigma-Aldrich
Iron, foil, thickness 0.1 mm, ≥99.9% trace metals basis
Sigma-Aldrich
Iron, powder, −325 mesh, 97%
Sigma-Aldrich
Iron, chips, 99.98% trace metals basis
Sigma-Aldrich
Ferritin from human spleen, Type V, 10 μg/mL in 0.15 M NaCl, 10 mM Tris, pH 8.0, containing 0.1% sodium azide
Sigma-Aldrich
Ferritin from equine spleen, Type I, saline solution
Iron, foil, light tested, 25x25mm, thickness 0.05mm, hard, 99.5%
Iron, foil, 6mm disks, thickness 0.5mm, hard, 99.5%
Iron, foil, 50mm disks, thickness 0.25mm, hard, 99.5%
Iron, foil, light tested, 100x100mm, thickness 0.025mm, as rolled, 99.99+%
Iron, foil, 8mm disks, thickness 0.05mm, as rolled, 99.99+%
Iron, foil, 6mm disks, thickness 0.125mm, hard, 99.5%
Iron, foil, not light tested, 100x100mm, thickness 0.005mm, 99.85%
Iron, foil, 50mm disks, thickness 0.38mm, hard, 99.5%
Iron, foil, light tested, 25x25mm, thickness 0.075mm, hard, 99.5%
Iron, foil, 6mm disks, thickness 0.020mm, 99.85%
Iron, foil, light tested, 100x100mm, thickness 0.025mm, hard, 99.5%
Iron, foil, 6mm disks, thickness 0.125mm, as rolled, 99.99+%
Iron, foil, 8mm disks, thickness 0.05mm, hard, 99.5%
Iron, foil, not light tested, 100x100mm, thickness 0.007mm, 99.85%
Iron, foil, 6mm disks, thickness 0.007mm, 99.85%
Iron, foil, 50mm disks, thickness 0.5mm, hard, 99.5%
Iron, foil, 8mm disks, thickness 0.020mm, 99.85%
Iron, foil, light tested, 300x300mm, thickness 0.075mm, hard, 99.5%
Iron, foil, 8mm disks, thickness 0.38mm, hard, 99.5%
Iron, foil, 8mm disks, thickness 0.003mm, 99.85%
Iron, foil, 6mm disks, thickness 0.004mm, 99.85%
Iron, foil, 50mm disks, thickness 0.1mm, hard, 99.5%
Iron, foil, light tested, 25x25mm, thickness 0.05mm, as rolled, 99.99+%