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Thermodynamic and structural characterization of the specific binding of Zn(II) to human protein DJ-1.

Thermodynamic and structural characterization of the specific binding of Zn(II) to human protein DJ-1.

Biochemistry (2014-04-05)

Shinya Tashiro, Jose M M Caaveiro, Chun-Xiang Wu, Quyen Q Hoang, Kouhei Tsumoto

ABSTRACT

Mutations of DJ-1 cause familial Parkinson's disease (PD), although the role of DJ-1 in PD remains unresolved. Very recent reports have shown that DJ-1 interacts with copper ions. This evidence opens new avenues to understanding the function of DJ-1 and its role in PD. Herein, we report that Zn(II) binds to DJ-1 with great selectivity among the other metals examined: Mn(II), Fe(II), Co(II), Ni(II), and Cu(II). High-resolution X-ray crystallography (1.18 Å resolution) shows Zn(II) is coordinated to the protein by the key residues Cys106 and Glu18. These results suggest that DJ-1 may be regulated and/or stabilized by Zn(II).

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Zinc, shot, <12 mm, 99.99% trace metals basis

Sigma-Aldrich

Zinc, pieces, 2-14 mesh, 99.9% trace metals basis

Sigma-Aldrich

Zinc preparation, 5 g/dL Zn⁺² in THF, highly reactive Rieke^®metal

Sigma-Aldrich

Zinc, foil, thickness 1.0 mm, 99.99% trace metals basis

Sigma-Aldrich

Zinc, granular, 20-30 mesh, ACS reagent, ≥99.8%

Sigma-Aldrich

Zinc, nanopowder, 40-60 nm avg. part. size, ≥99% trace metals basis

Sigma-Aldrich

Zinc, sticks, diam. 7-10 mm, 99.97% trace metals basis

Zinc, foil, 10mm disks, thickness 0.025mm, as rolled, 99.95+%

Zinc, foil, 15mm disks, thickness 0.005mm, 99.9%

Zinc, foil, 50x50mm, thickness 0.15mm, as rolled, 99.95+%

Zinc, foil, 15mm disks, thickness 0.125mm, as rolled, 99.99+%

Zinc, foil, 10mm disks, thickness 0.02mm, 99.9%

Zinc, foil, 0.5m coil, thickness 0.05mm, as rolled, 99.95+%

Zinc, foil, 25mm disks, thickness 0.025mm, as rolled, 99.99+%

Zinc, foil, 0.5m coil, thickness 0.025mm, as rolled, 99.99+%

Zinc, foil, 15mm disks, thickness 1.0mm, as rolled, 99.99+%

Zinc, foil, 10mm disks, thickness 0.25mm, as rolled, 99.99+%

Zinc, foil, 50x50mm, thickness 0.1mm, as rolled, 99.95+%

Zinc, foil, 25mm disks, thickness 0.05mm, as rolled, 99.95+%

Zinc, foil, 25mm disks, thickness 0.15mm, as rolled, 99.95+%

Zinc, foil, 0.5m coil, thickness 0.125mm, as rolled, 99.95+%

Zinc, foil, 15mm disks, thickness 0.1mm, as rolled, 99.95+%

Zinc, foil, 10mm disks, thickness 0.35mm, as rolled, 99.95+%

Zinc, foil, 0.3m coil, thickness 0.175mm, as rolled, 99.95+%

Zinc, foil, 25mm disks, thickness 0.05mm, as rolled, 99.99+%

Zinc, foil, 25mm disks, thickness 0.175mm, as rolled, 99.95+%

Zinc, foil, 15mm disks, thickness 0.25mm, as rolled, 99.99+%

Zinc, foil, 1m coil, thickness 0.025mm, as rolled, 99.95+%

Zinc, foil, 1m coil, thickness 0.075mm, as rolled, 99.95+%

Zinc, foil, 10mm disks, thickness 0.125mm, as rolled, 99.95+%