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  • Aptamer-targeted hyperbranched polymers: towards greater specificity for tumours in vivo.

Aptamer-targeted hyperbranched polymers: towards greater specificity for tumours in vivo.

Chemical communications (Cambridge, England) (2013-02-19)
Daniel J Coles, Barbara E Rolfe, Nathan R B Boase, Rakesh N Veedu, Kristofer J Thurecht
ABSTRACT

Hyperbranched polymers conjugated to a peptide-aptamer were prepared using a combination of RAFT polymerisation and click chemistry for targeting tumour cells in vivo. The polymers showed enhanced cell-uptake in vitro (compared to unconjugated polymer) while excellent specificity for solid tumours was observed in vivo using a mouse model of melanoma.

MATERIALS
Product Number
Brand
Product Description

Supelco
Rhodamine B solution, 0.2% in isopropanol, for TLC derivatization
Sigma-Aldrich
Rhodamine B, ≥95% (HPLC)
Sigma-Aldrich
Rhodamine B, for fluorescence
Supelco
Rhodamine B, analytical standard