SAB4200657

Monoclonal Anti-EEF1A1 antibody produced in mouse

clone EF48, purified from hybridoma cell culture

• Synonym(s): CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, GRAF-1EF, HNGC:16303, LENG7, PTI1, eEF1A-1, eukaryotic translation elongation factor 1 α 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: EF48
• form: buffered aqueous solution
• species reactivity: bovine, mouse, rat, human, monkey, canine
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 1-2 μg/mL using whole extracts of HEK-293T cells.; immunofluorescence: 0.5-1 μg/mL using SW-620 cells.
• isotype: IgG2b
• UniProt accession no.: P68104
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EEF1A1(1915) ; mouse ... Eef1a1(13627) ; rat ... Eef1a1(171361)

Description

General description

Eukaryotic translation elongation factor 1 α 1 (EEF1A1) is a cellular protein which is part of the eukaryotic elongation factor 1 (eEF1) complex. The gene encoding it is localized on human chromosome 6q14.1.

Monoclonal Anti-EEF1A1 (mouse IgG2b isotype) is derived from the hybridoma EF48 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Eukaryotic elongation factor 1 A-1 (EEF1A1), also known as EF-l α is a GTP-binding protein. EEF1A1 is one of the most abundant cytoplasmic proteins and is ubiquitously expressed except in neurons and muscle.

Immunogen

a synthetic peptide corresponding to an internal sequence of human EEF1A1

Application

Monoclonal Anti-EEF1A1 antibody produced in mouse has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Eukaryotic elongation factor 1 A-1 (EEF1A1) is involved in proper codon-anticodon binding interactions between mRNAs and tRNAs at the tRNA acceptor site of the ribosome. EEF1A also plays a translation-independent role in embryogenesis, senescence, oncogenic transformation, cell proliferation, apoptosis, cytoskeletal organization and protein degradation. Overexpression of EEF1A1 has been observed in various tumors of the pancreas, colon, breast, lung, and stomach.

Eukaryotic translation elongation factor 1 α 1 (EEF1A1) binds to aminoacetylated tRNAs and delivers them to the elongating ribosome during protein synthesis. It aids in the association of the eukaryotic elongation factor 1 (eEF1) complex with the HIV-1 (human immunodeficiency virus-1) reverse transcription complex. EEF1A1 also associates with genomic RNA secondary structures of RNA viruses and helps in their replication.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable