OP60
Anti-Bcl-2 (Ab-1) Mouse mAb (100)
Synonym(s):
Anti-Bcl-2α
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About This Item
antibody form
purified antibody
Quality Level
clone
100, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
should not react with
mouse
isotype
IgG1
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-AG14 plasmacytoma cells (see application references). Recognizes the ~24-26 kDa bcl-2 protein.
Recognizes the ~24-26 kDa Bcl-2 protein in MCF-7 cells, tonsil, and lymph node tissue.
The bcl-2 proto-oncogene is encoded by a gene 230 kb in size that gives rise to a ~24-26 kDa protein which has been localized to the mitochondrial membrane. Although some data exists for implicating bcl-2 as a novel GTP binding protein, the major function of bcl-2 appears to be an inhibitor of apoptosis or "programmed cell death". It has now been well documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore constitutively activated in B cell malignancies (i.e. follicular lymphoma and chronic lymphocytic leukemia). In addition, bcl-2 has also been shown to protect mammalian cells from sodium azide, colchicine, DNA, RNA, and protein-synthesis inhibitors, as well as steroids, heat shock and irradiation.
Immunogen
a synthetic peptide (GAAPAPGIFSSQPG) corresponding to amino acids 41-54 of human Bcl-2
Application
Frozen Sections (1-5 μg/ml)
Immunoblotting (1-5 μg/ml)
Paraffin Sections (1-10 μg/ml, see comments)
Immunoblotting (1-5 μg/ml)
Paraffin Sections (1-10 μg/ml, see comments)
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Analysis Note
Positive Control
MCF7 cells or tonsil or lymph node tissue
MCF7 cells or tonsil or lymph node tissue
Other Notes
Generally, no pre-treatment of paraffin sections is needed, but treating with heat may improve staining in some tissues. Antibody should be titrated for optimal results in individual sample types.
Certificates of Analysis (COA)
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J C Reed et al.
Molecular and cellular biology, 10(8), 4370-4374 (1990-08-01)
The BCL2 (B cell lymphoma/leukemia-2) and C-HA-RAS oncogenes encode membrane-associated proteins of 26 and 21 kilodaltons, respectively. Although RAS proteins have long been known for their ability to bind and hydrolyze GTP, recent investigations suggest that BCL2 encodes a novel
D L Vaux
Proceedings of the National Academy of Sciences of the United States of America, 90(3), 786-789 (1993-02-01)
Cell death is a normal physiological process. Morphological studies have shown that cells that die by physiological mechanisms often undergo characteristic changes termed "apoptosis" or "programmed cell death." Recent work has begun to unravel the molecular mechanisms of these deaths
G A Silverman et al.
Molecular and cellular biology, 13(9), 5469-5478 (1993-09-01)
The goal of this study was to determine whether it will be feasible to study the expression of a large, human gene, such as the BCL2 proto-oncogene, by DNA transfection. The BCL2 proto-oncogene is 230 kb in size and is
A J Wagner et al.
Molecular and cellular biology, 13(4), 2432-2440 (1993-04-01)
The product of the c-myc proto-oncogene is an important positive regulator of cell growth and proliferation. Recently, c-Myc has also been demonstrated to be a potent inducer of apoptosis when expressed in the absence of serum or growth factors. To
G Nunez et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(12), 4589-4593 (1989-06-01)
Human follicular B-cell lymphomas possess a t(14;18) that translocates a putative protooncogene, BCL2, into the immunoglobulin heavy chain locus. The normal BCL2 gene is quiescent in resting B cells, expressed in proliferating, but down-regulated in differentiated B cells. Inappropriately high
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