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MAB4419

Sigma-Aldrich

Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2

clone 7F9.2, from mouse

Synonym(s):

Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7F9.2, monoclonal

species reactivity

human, mouse

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

input

sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

isotype

IgG1κ

NCBI accession no.

NP_976034

UniProt accession no.

P20263

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... POU5F1(5460)
mouse ... Pou5F1(18999)

General description

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.

Specificity

Reactivity to other species has not been determined.
This antibody reacts with the intracellular protein, Oct-4 and detects the Oct-4A isoform.

Immunogen

Recombinant human Oct-4 protein, a.a. 1-145.

Application

Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2 is validated for use in IC, FC, WB for the detection of OCT-4 [POU5F1].
Western Blotting: Recommended working dilution is 1 - 2 µg/mL.

Flow Cytometry: Recommended working dilution is 2 µg/mL.

Immunocytochemistry: Recommended working dilution is 1:100 – 1: 200.

Target description

~39 kDa

Linkage

Replaces: MAB4305

Physical form

Format: Purified
Protein G sepharose column
Purified mouse monoclonal IgG1κ in a buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% NaN3.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pushpa Singh et al.
Stem cell research & therapy, 13(1), 60-60 (2022-02-07)
True identity and specific set of markers to enrich endometrial stem cells still remains elusive. Present study was undertaken to further substantiate that very small embryonic-like stem cells (VSELs) are the true and elusive stem cells in adult mice endometrium.
Gisele C M Dias et al.
Molecular reproduction and development (2020-06-20)
The objective of this study was to establish a protocol for the characterization, isolation, and culture of type A spermatogonia using specific molecular markers for these cells in fish. To this end, adult Prochilodus lineatus testes were collected and digested
Molecular signatures to define spermatogenic cells in common marmoset (Callithrix jacchus).
Lin, ZY; Imamura, M; Sano, C; Nakajima, R; Suzuki, T; Yamadera, R; Takehara, Y; Okano et al.
Reproduction (Cambridge, England) null
Teresa Starzyńska et al.
Journal of cellular and molecular medicine, 17(6), 792-799 (2013-05-16)
Various experimental studies indicate potential involvement of bone marrow (BM)-derived stem cells (SCs) in malignancy development and progression. In this study, we comprehensively analysed systemic trafficking of various populations of BM-derived SCs (BMSCs), i.e., mesenchymal, haematopoietic, endothelial stem/progenitor cells (MSCs
Foster Birnbaum et al.
NPJ Regenerative medicine, 7(1), 19-19 (2022-03-20)
Duchenne muscular dystrophy (DMD) is a progressive genetic myopathy that leads to heart failure from dilated cardiomyopathy by early adulthood. Recent evidence suggests that tamoxifen, a selective estrogen receptor modulator widely used to treat breast cancer, ameliorates DMD cardiomyopathy. However
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