MAB1217-I

Anti-Fmc-7 (B-Cell Lymphocyte Marker), clone Fmc-7 Antibody

clone Fmc-7, from mouse

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: Fmc-7, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable; western blot: suitable
• isotype: IgMκ
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MS4A1(931)

Description

General description

B cells are lymphocytes that play a large role in the humoral immune response as opposed to the cell-mediated immune response that is governed by T cells. The principal function of B cells is to make antibodies against soluble antigens. B cells are an essential component of the adaptive immune system. FMC-7 B-Cell Lymphocyte Marker is a 105 kDa B cell restricted antigen which is expressed on about 50% of adult human peripheral blood B cells. Upon in vivo B cell activation FMC7 expression initially increases. B cells involved in antibody secretion have lost the FMC7 determinant. The monoclonal antibody FMC7 delineates a subpopulation of B lymphocytes in normal blood. Expression of the antigen recognized by FMC7 appears to be maturation-linked, and it serves to distinguish different types of B cell leukemia.

Immunogen

HRIK [human B-lymphoblastoid cell] corresponding to human Fmc-7 (B-Cell Lymphocyte Marker).

Application

Flow Cytometry Analysis: 4.0 µg of this antibody detected Fmc-7 (B-Cell Lymphocyte Marker) in peripheral blood mononuclear cells.

This Anti-Fmc-7 (B-Cell Lymphocyte Marker) antibody is validated for use in WB, FC for the detection of Fmc-7 (B-Cell Lymphocyte Marker).

Quality

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Fmc-7 (B-Cell Lymphocyte Marker) in 10 µg of THP-1 cell lysate.

Target description

~105 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing 0.1M Tris, 10mM Glycine, 0.5M NaCl, and 0.1% Sodium Azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable