HPLC Analysis of Creatine Kinase on Discovery® BIO PolyMA-WAX
CONDITIONS
column
Discovery BIO PolyMA-SCX, 5 cm × 4.6 mm, 5 μm particles (59601-U)
mobile phase
[A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5M NaCl, pH 7.0
flow rate
0.5 mL/min
injection
10 μL
column temp.
25 °C
detector
UV, 280 nm
Description
Analysis Note
Discovery BIO PolyMA-SCX and PolyMA-WAX columns give sharp, efficient peaks for a wide variety of proteins.
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany