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Key Documents

04-811

Sigma-Aldrich

Anti-phospho-eNOS/NOS III (Thr495) Antibody, rabbit monoclonal

culture supernatant, Upstate®

Synonym(s):

NOS III, endothelial nitric oxidase synthase, endothelial nitric oxide synthase, nitric oxide synthase 3 (endothelial cell)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

monoclonal

species reactivity

bovine

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_000603

UniProt accession no.

P29474

shipped in

dry ice

target post-translational modification

phosphorylation (pThr495)

Gene Information

mouse ... Nos3(287024)

Specificity

Predicted cross-reactivity with human, rabbit, mouse and rat
Recognizes phosphorylated eNOS/NOS III.

Immunogen

Peptide containing the sequence KK[pT]FK in which pT corresponds to phosphothreonine at residue 495 of human eNOS/NOS III

Application

This Anti-phospho-eNOS/NOS III (Thr495) Antibody is validated for use in WB for the detection of phospho-eNOS/NOS III (Thr495).
Western Blot Analysis:
A 1:2,000-1:10,000 dilution of this lot detected phosphorylated eNOS/NOS III in fetal bovine heart endothelial cell lysate.

Quality

routinely evaluated by immunoblot on fetal bovine heart endothelial cell lysate

Target description

135 kDa

Linkage

Replaces: 05-811

Physical form

Cultured supernatant in 0.05% sodium azide

Analysis Note

Control
Fetal bovine heart endothelial cell lysate

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Novel protective role of endogenous cardiac myocyte P2X4 receptors in heart failure.
Yang, T; Shen, JB; Yang, R; Redden, J; Dodge-Kafka, K; Grady, J; Jacobson, KA; Liang, BT
Circulation. Heart Failure null
A P S Mesquita et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 55, e11612-e11612 (2022-02-10)
Anoikis is a type of apoptosis that occurs in response to the loss of adhesion to the extracellular matrix (ECM). Anoikis resistance is a critical mechanism in cancer and contributes to tumor metastasis. Nitric oxide (NO) is frequently upregulated in
Martina Monti et al.
The Journal of pharmacology and experimental therapeutics, 351(3), 500-509 (2014-09-23)
At the cardiovascular level, nitric oxide (NO) controls smooth muscle functions, maintains vascular integrity, and exerts an antihypertensive effect. Metal-nonoates are a recently discovered class of NO donors, with NO release modulated through the complexation of the N-aminoethylpiperazine N-diazeniumdiolate ligand

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