Skip to Content
Merck
  • A role for EIIA(Ntr) in controlling fluxes in the central metabolism of E. coli K12.

A role for EIIA(Ntr) in controlling fluxes in the central metabolism of E. coli K12.

Biochimica et biophysica acta (2013-07-28)
Susan Jahn, Bart R Haverkorn van Rijsewijk, Uwe Sauer, Katja Bettenbrock
ABSTRACT

To investigate a possible role of the nitrogen-PTS (PTS(Ntr)) in controlling carbon metabolism, we determined the growth of Escherichia coli LJ110 and of isogenic derivatives, mutated in components of the PTS(Ntr), on different carbon sources. The PTS(Ntr) is a set of proteins homologous to the PEP-dependent phosphotransferase system (C-PTS) that transfers a phosphate group from PEP over EI(Ntr) (encoded by ptsP) and NPr (encoded by ptsO) to EIIA(Ntr) (encoded by ptsN). Strains deleted in ptsN were characterized by a high acetate production coupled to slow growth on glycolytic substrates. The ΔptsP and the ΔptsO strain showed the same behavior as the parent strain. As the phosphorylation level of EIIA(Ntr) in these mutants differed significantly from that of the parent strain, phosphorylation of EIIA(Ntr) obviously is not important for its function. During growth in minimal medium with defined carbon sources, EIIA(Ntr) was always completely phosphorylated in LJ110. Significant amounts of dephosphorylated EIIA(Ntr) were only visible in strains lacking EI(Ntr) or NPr. mRNA expression studies on glucose revealed a downregulation of genes encoding TCA cycle enzymes when EIIA(Ntr) was absent. (13)C-flux analyses confirmed higher fluxes towards acetate and lower fluxes in the TCA cycle in the ptsN mutants but additionally hinted to a slightly but significantly increased flux through the pyruvate dehydrogenase complex (PDH). During growth on succinate the ΔptsN strain accumulated mutations in rpoS, while no rpoS mutants were observed for the ΔptsN-O strain. This hints to an additional function of NPr during growth with succinate.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Succinic acid, puriss. p.a., ACS reagent, ≥99.5% (T)
Sigma-Aldrich
Succinic acid, ACS reagent, ≥99.0%
Sigma-Aldrich
Succinic acid, purum p.a., ≥99.0% (T)
Sigma-Aldrich
Succinic acid, BioXtra, BioRenewable, ≥99.0%
Sigma-Aldrich
Succinic acid, ReagentPlus®, BioRenewable, ≥99.0%
Supelco
Succinic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Succinic acid, BioReagent, BioRenewable, suitable for cell culture, suitable for insect cell culture