Simultaneous detection of SERS and fluorescence using a single excitation for microbead-based analysis.

Simultaneous detection of SERS and fluorescence using a single excitation for microbead-based analysis.

Journal of biomedical nanotechnology (2013-08-06)

Sa Ram Lee, Chang Su Jeon, Inseong Hwang, Taek Dong Chung, Hee Chan Kim

ABSTRACT

We demonstrate simultaneous detection of surface-enhanced Raman scattering (SERS) and fluorescence signals from a silver microbead. For the dual signal generation, silver microbeads with a diameter of 15 microm were functionalized with benzenethiol (BT) as a Raman tag and a cardiac troponin I (cTnI) antibody on their surface. SERS and fluorescence signals were obtained using a single argon laser source with 488 nm wavelength. The SERS signals from Raman tag can be used as identification indices for decoding a particular microbead, while the fluorescence signals provide the information about molecular interactions with a specific biomarker. With simultaneous detection of SERS and fluorescence signals using single excitation on the functionalized microbeads, we successfully showed the possibility of a simple barcoding strategy for multiplex analysis using suspension arrays.

MATERIALS

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