- Activity of Debaryomyces hansenii UFV-1 α-galactosidases against α-D-galactopyranoside derivatives.
Activity of Debaryomyces hansenii UFV-1 α-galactosidases against α-D-galactopyranoside derivatives.
Carbohydrate research (2011-02-25)
Pollyanna A Viana, Sebastião T de Rezende, Arianne de A Alves, Rozângela M Manfrini, Ricardo J Alves, Marcelo P Bemquerer, Marcelo M Santoro, Valéria M Guimarães
PMID21345419
ABSTRACT
α-D-Galactopyranosides were synthesized and their inhibitory activities toward the Debaryomyces hansenii UFV-1 extracellular and intracellular α-galactosidases were evaluated. Methyl α-D-galactopyranoside was the most potent inhibitor compared to the others tested, with K(i)(') values of 0.82 and 1.12 mmolL(-1), for extracellular and intracellular enzymes, respectively. These results indicate that the presence of a hydroxyl group in the C-6 position of α-D-galactopyranoside derivatives is important for the recognition by D. hansenii UFV-1 α-galactosidases.
MATERIALS