Skip to Content
Merck
All Photos(1)

Key Documents

L1887

Sigma-Aldrich

Lipopolysaccharides from Salmonella enterica serotype abortus equi

purified by gel-filtration chromatography

Synonym(s):

LPS

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00131520
UNSPSC Code:
12352201
NACRES:
NA.25

biological source

Salmonella enterica (Serotype abortus equi)

form

lyophilized powder

purified by

gel-filtration chromatography

impurities

<1% Protein

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

This product is extracted and purified by gel filtration from Salmonella abortus equi and purified by gel filtration. The source strain is ATCC 9842. Lipopolysaccharide from Salmonella abortus equi stimulates human mononuclear cells to produce tumor necrosis factor. This LPS is also mitogenic for frog and mouse lymphocytes.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H300

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Kanchan Phadwal et al.
Autophagy, 8(4), 677-689 (2012-02-04)
Autophagy is a conserved constitutive cellular process, responsible for the degradation of dysfunctional proteins and organelles. Autophagy plays a role in many diseases such as neurodegeneration and cancer; however, to date, conventional autophagy detection techniques are not suitable for clinical
Matthew Hoffmann et al.
Xenobiotica; the fate of foreign compounds in biological systems, 41(12), 1063-1075 (2011-08-24)
Apremilast is a novel, orally available small molecule that specifically inhibits PDE4 and thus modulates multiple pro- and anti-inflammatory mediators, and is currently under clinical development for the treatment of psoriasis and psoriatic arthritis. The pharmacokinetics and disposition of [(14)C]apremilast
Tao Ni et al.
Science advances, 6(5), eaax8286-eaax8286 (2020-02-18)
Perforin-2 (MPEG1) is thought to enable the killing of invading microbes engulfed by macrophages and other phagocytes, forming pores in their membranes. Loss of perforin-2 renders individual phagocytes and whole organisms significantly more susceptible to bacterial pathogens. Here, we reveal
Pau Gonzalez et al.
Neuroscience research, 114, 16-29 (2016-08-27)
Despite the essential functions of astrocytes and the emerging relevance of the Wnt family of proteins in the CNS under physiological and pathological conditions, the astroglial expression of this family of proteins and its potential modulatory role on astroglial activation

Related Content

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service