SAB4200361
Anti-LC3B antibody, Mouse monoclonal
clone LC3B-6, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-ATG8F, Monoclonal Anti-LC3, Monoclonal Anti-MAP1A/1BLC3, Monoclonal Anti-MAP1LC3B, Monoclonal Anti-microtubule-associated protein 1 light chain 3 beta
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All Photos(2)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
LC3B-6, monoclonal
form
buffered aqueous solution
mol wt
antigen ~18/16 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 10-20 μg using lysates of human U-87 cells.
western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over-expressing human LC3B.
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MAP1LC3B(81631)
General description
The gene MAP1LC3B (microtubule associated protein 1 light chain 3 β) is a homolog of ATG8 gene, an autophagy-related gene in yeast. The encoded protein is called pro-MAP1LC3B, a pro-form that is cleaved by ATG4 to produce a cytosolic form called MAP1LC3B-I. Anti-LC3B antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma LC3B-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the N-terminal region of human LC3B.
Immunogen
synthetic peptide corresponding to the N-terminal region of human LC3B, conjugated to KLH. The corresponding sequence differs by one amino-acid in rat and mouse.
Application
Anti-LC3B antibody produced in rabbit has been used in:
- western blotting
- immunofluorescence
- confocal laser microscopy
- immunoprecipitation
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Biochem/physiol Actions
MAP1LC3B-I (microtubule associated protein 1 light chain 3 β-I) conjugates with MAP1LC3B-II before being inserted into both inner and outer membranes of the growing autophagic vesicle during autophagy. The cellular distribution and processing of MAP1LC3B facilitates monitoring of autophagy. Cells that lack MAP1LC3B are found to be defective in the formation of autophagic vesicles, with altered phenotypes associated with the role of autophagy in energy homeostasis and removal of protein aggregates. It can serve as an autophagic marker.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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