P0065
Phospholipase D from Streptomyces chromofuscus
≥50,000 units/mL, buffered aqueous glycerol solution
Synonym(s):
Lecithinase D, Phosphatidylcholine phosphatidohydrolase
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
form
buffered aqueous glycerol solution
specific activity
≥50,000 units/mL
mol wt
~60 kDa
shipped in
dry ice
storage temp.
−70°C
Looking for similar products? Visit Product Comparison Guide
General description
Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.
Application
Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs).
Biochem/physiol Actions
Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline. PC-PLD activity is regulated by hormones, cytokines, neurotransmitters and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway. PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis.
Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Unit Definition
One unit will release 1 μmol of choline from L-α-phosphatidylcholine per hour at pH 8.0 and 30 °C.
Physical form
Solution in 100 mM Tris/HCl, pH 8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v).
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Phospholipase D: a downstream effector of ARF in granulocytes.
Cockcroft S
Science, 263(5146), 523-526 (1994)
Dynamics and function of phospholipase D and phosphatidic acid during phagocytosis.
Traffic, 7(3), 365-377 (2006)
Lecithin: a by-product of biodiesel production and a source of choline for dairy cows
Italian Journal of Animal Science, 11:2 (2012)
Phospholipase D: enzymology, mechanisms of regulation, and function.
Exton JH
Physiological Reviews, 77(2), 303-320 (1997)
Eliminating office barriers to the handicapped patient.
Henson JL
The New dentist, 9(6), 30-32 (1979)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service