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O4264

Sigma-Aldrich

Human Osteopontin

recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

Synonym(s):

Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)

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About This Item

MDL number:
MFCD03100698
UNSPSC Code:
12352202
NACRES:
NA.32

product name

Osteopontin human, recombinant, expressed in NSO cells, ≥95% (SDS-PAGE), lyophilized powder, suitable for cell culture

biological source

human

Quality Level

100

recombinant

expressed in NSO cells

sterility

sterile

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

60-65 kDa by SDS-PAGE (reducing)

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P10451

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... SPP1(6696)

General description

Secreted phosphoprotein 1 (SPP1) or osteopontin is a secreted cytokine. This phosphorylated acidic glycoprotein is expressed in various cells and tissues. It is synthesized by osteoblasts. The gene encoding SPP1 is localized on human chromosome 4q22.1.

Application

Anti-Osteopontin antibody produced in rabbit has been used for ELISA.

Biochem/physiol Actions

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity. Secreted phosphoprotein 1 (SPP1) or osteopontin modulates inflammatory responses and glucose metabolism. It is a chemotactic factor and has a role in immune cell recruitment. The protein is also associated with cell growth, migration and differentiation. SPP1 attaches osteoclasts to the bone matrix. It is also involved in atherosclerotic process and bone mineralization. It is a marker of many cancers.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to mediate 293 cell adhesion.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Polycystic ovary syndrome is associated with increased osteopontin levels
European Journal of Endocrinology (2015)
Expanded comparative mapping between man
and rabbit and detection of a new conserved
segment between HSA22 and OCU4
C Chantry
Cytogenetic and genome research (2005)
Osteopontin Fragments with Intact Thrombin-Sensitive Site Circulate in Cervical Cancer Patients
Danny TM
PLoS ONE (2016)
Comparison of Plasma Osteopontin Levels between Patients with Borderline Ovarian Tumours and Serous Ovarian Carcinoma.
Zivny JH
Folia Biologica (Praha.) (2016)
Suhail Tahir et al.
Journal of immunology (Baltimore, Md. : 1950), 194(12), 5725-5735 (2015-05-15)
Immune aging results in diminished adaptive immunity and increased risk for autoimmunity. We previously reported a unique PD-1(+) CD44(high)CD4(+) T cell population that increases with age in normal mice. In this study, we indicate that the age-dependent PD-1(+) CD44(high)CD4(+) T
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