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1.51468

Supelco

Chromolith® RP-8 endcapped (monolithic) HPLC Columns

L × I.D. 100 mm x 4.6 mm HPLC Column

Synonym(s):

Chromolith® Performance RP-8 endcapped 100-4.6 HPLC column

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

Chromolith® RP-8 endcapped, L × I.D. 100 mm x 4.6 mm HPLC column

material

PEEK column

Quality Level

Agency

suitable for USP L7

product line

Chromolith®

feature

endcapped

packaging

1 ea of

extent of labeling

11% Carbon loading

parameter

200 bar pressure
45 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

10 cm × 4.6 mm

matrix

monolithic silica

matrix active group

C8 bonding phase

pore size

13 nm mesoporosity
2 μm macroporosity

operating pH range

2-7.5

separation technique

reversed phase

storage temp.

no temp limit

General description

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® Performance RP-8 endcapped columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is Octylsilane with full end-capping. Chromolith® Performance RP-8 endcapped columns fall under the USP L7 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® Performance RP-8 endcapped columns are suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for fast separations of complex samples on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® RP-8 endcapped 5-4.6 guard cartridges (3 pieces) [1.52013] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Analysis Note

Carbon content: 10.4 - 11.9 %
Pressure: ≤ 30 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 2.0 - 2.5
Tailing (USP): 0.90 - 1.60 %
Selectivity
separation factor (anthracene/progesterone): 1.40 - 1.75

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

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