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  • Clinical significance of joint detection of serum VEGF, SIL-2R and HGF in patients with primary hepatocellular carcinoma before and after percutaneous microwave coagulation therapy.

Clinical significance of joint detection of serum VEGF, SIL-2R and HGF in patients with primary hepatocellular carcinoma before and after percutaneous microwave coagulation therapy.

Asian Pacific journal of cancer prevention : APJCP (2014-06-28)
Ji-Dong Chen, Yan-Qun Xiong, Ke Dong, Jun Luo, Lin-Xian Yue, Qin Chen
ABSTRACT

To investigate the changes of serum vascular endothelial growth factor (VEGF), soluble interleukin-2 receptor (SIL-2R) and hepatocyte growth factor (HGF) contents in patients with primary hepatocellular carcinoma (HCC) before and after percutaneous microwave coagulation therapy (PMCT) and determine their clinical significance. Fasting venous blood (3 mL) from 81 patients with primary HCC diagnosed by pathology was collected in the mornings 1 day before PMCT, and 1 day, 7 days and 1 month after PMCT, and then the serum was separated and stored in -70°. The contents of VEGF, SIL-2R and HGF were detected by enzyme linked immunosorbent assay (ELISA). The serum VEGF, SIL-2R and HGF contents in 81 patients with primary HCC had obviously dynamic changes before and after PMCT. By comparison to 1 day after PMCT with pre-operation, there was no statistical significance regarding VEGF and SIL-2R contents (P>0.05), but HGF content showed significant difference (P<0.01). Compared with pre-operation, VEGF, SIL-2R and HGF contents 7 days and 1 month after PMCT all manifested significant differences (P<0.01). By comparison to 7 days with 1 month after PMCT, there was no statistical significance regarding the VEGF content (P>0.05), whereas SIL-2R and HGF contents showed significant change (P<0.01). The contents of serum VEGF, SIL-2R and HGF have obviously dynamic changes in primary HCC before and after PMCT, and their joint detection is expected to be an effective hematologic evaluation index of PMCT for primary HCC.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Phosphotyrosine MET / Hepatocyte Growth Factor Receptor ELISA Kit
Sigma-Aldrich
Human MET / Hepatocyte Growth Factor Receptor ELISA Kit
Sigma-Aldrich
Mouse HGF ELISA Kit, for serum, plasma and cell culture supernatant
Sigma-Aldrich
Mouse HGF ELISA Kit, for cell and tissue lysates
Sigma-Aldrich
Human HGF ELISA Kit, for serum, plasma, cell culture supernatant and urine
Sigma-Aldrich
Human HGF ELISA Kit, for cell and tissue lysates