Skip to Content

The uptake of ZnO and CuO nanoparticles in the water-flea Daphnia magna under acute exposure scenarios.

The uptake of ZnO and CuO nanoparticles in the water-flea Daphnia magna under acute exposure scenarios.

Environmental pollution (Barking, Essex : 1987) (2014-08-12)

Nathalie Adam, Frédéric Leroux, Dries Knapen, Sara Bals, Ronny Blust

ABSTRACT

In this study the uptake of ZnO and CuO nanoparticles by Daphnia magna was tested. Daphnids were exposed during 48 h to acute concentrations of the nanoparticles and corresponding metal salts. The Daphnia zinc and copper concentration was measured and the nanoparticles were localized using electron microscopy. The aggregation and dissolution in the medium was characterized. A fast dissolution of ZnO in the medium was observed, while most CuO formed large aggregates and only a small fraction dissolved. The Daphnia zinc concentration was comparable for the nanoparticles and salts. Contrarily, a much higher Daphnia copper concentration was observed in the CuO exposure, compared to the copper salt. CuO nanoparticles adsorbed onto the carapace and occurred in the gut but did not internalize in the tissues. The combined dissolution and uptake results indicate that the toxicity of both nanoparticle types was caused by metal ions dissolved from the particles in the medium.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Copper, beads, 2-8 mm, 99.9995% trace metals basis

Sigma-Aldrich

Copper, foil, thickness 1.0 mm, 99.999% trace metals basis

Sigma-Aldrich

Copper, wire, diam. 0.25 mm, 99.999% trace metals basis

Sigma-Aldrich

Copper, foil, thickness 0.5 mm, 99.98% trace metals basis

Sigma-Aldrich

Copper, beads, 2-8 mm, ≥99.99% trace metals basis

Continuous cast copper (O), BCR^®, certified reference material, rod

Sigma-Aldrich

Copper, foil, thickness 0.025 mm, 99.98% trace metals basis

Sigma-Aldrich

Copper, wire, diam. 1.0 mm, ≥99.9%

Sigma-Aldrich

Copper, powder, <75 μm, 99%

Sigma-Aldrich

Copper, wire, diam. 0.64 mm, 99.995% trace metals basis

Sigma-Aldrich

Copper, wire, diam. 2.0 mm, 99.999% trace metals basis

Sigma-Aldrich

Copper, foil, ≥99.8% (complexometric)

Copper - O.F.H.C., foil, 50mm disks, thickness 0.25mm, half hard, 99.95+%

Copper, wire reel, 100m, diameter 0.025mm, as drawn, 99.99+%

Copper - O.F.H.C., foil, 25mm disks, thickness 0.7mm, half hard, 99.95+%

Copper - O.F.H.C., foil, 50mm disks, thickness 0.5mm, half hard, 99.95+%

Copper - O.F.H.C., foil, 6mm disks, thickness 0.006mm, hard, 99.95+%

Copper - O.F.H.C., foil, 2m coil, thickness 0.125mm, hard, 99.95+%

Copper - O.F.H.C., foil, 25mm disks, thickness 1.0mm, half hard, 99.95+%

Copper, wire reel, 5m, diameter 0.5mm, as drawn, 99.98+%

Copper - O.F.H.C., foil, 2m coil, thickness 0.7mm, half hard, 99.95+%

Copper, wire reel, 50m, diameter 0.25mm, as drawn, 99.99+%

Copper, wire reel, 500m, diameter 0.125mm, annealed, 99.9%

Copper, wire reel, 500m, diameter 0.05mm, annealed, 99.9%

Copper, wire reel, 20m, diameter 0.5mm, as drawn, 99.999%

Copper - O.F.H.C., foil, 50mm disks, thickness 0.010mm, hard, 99.95+%

Copper - O.F.H.C., foil, 50mm disks, thickness 0.05mm, hard, 99.95+%

Copper, wire reel, 2m, diameter 0.75mm, as drawn, 99.98+%

Copper, wire reel, 1m, diameter 0.75mm, as drawn, 99.98+%

Copper, wire reel, 500m, diameter 0.15mm, annealed, silver plated, 99.9%