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  • Dynamic investigation of interaction of biocompatible iron oxide nanoparticles with epithelial cells for biomedical applications.

Dynamic investigation of interaction of biocompatible iron oxide nanoparticles with epithelial cells for biomedical applications.

Journal of biomedical nanotechnology (2013-08-29)
Alice Panariti, Barbara Lettiero, Rodica Alexandrescu, Maddalena Collini, Laura Sironi, Munish Chanana, Ion Morjan, Dayan Wang, Giuseppe Chirico, Giuseppe Miserocchi, Cecilia Bucci, Ilaria Rivolta
ABSTRACT

Magnetic nanoparticles have emerged as important players in current research in modern medicine since they can be used in medicine for diagnosis and/or therapeutic treatment of diseases. Among many therapeutic applications of iron-based nanoparticles, drug delivery and photothermal therapy are of particular interest. At cellular level their uptake has been studied and the mechanism by which nanoparticles enter into the cell has important implication not only for their fate but also for their impact on the biological systems. We present here a dynamic investigation of interaction of biocompatible iron oxide nanoparticles coated with L-3,4-dihydroxyphenylalanine and labeled with tetra-methylrhodamine-5/6-isothiocyanate with lung epithelial cells. Our data show that after macropinocytosis-mediated internalization, nanoparticles in form of vesicles approach the nucleus and converge in the more acidic compartments of the cells in a microtubule-dependent manner. During progression the nanoparticles aggregate. Finally, we have demonstrated that a converging laser radiation on the cells, causes the increase in the local temperature and thus damages the cells, suggesting that these nanoparticles may be applied for photothermal therapy studies.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Iron(III) oxide, ≥99.995% trace metals basis
Sigma-Aldrich
Iron(III) oxide, nanopowder, <50 nm particle size (BET)
Sigma-Aldrich
Iron(III) oxide, powder, <5 μm, ≥96%