Skip to Content
Merck
  • High performance liquid chromatography-tandem mass spectrometry method for residue determination of 39 plant growth regulators in root and rhizome Chinese herbs.

High performance liquid chromatography-tandem mass spectrometry method for residue determination of 39 plant growth regulators in root and rhizome Chinese herbs.

Food chemistry (2020-04-20)
Chayanis Sutcharitchan, Shui Miao, Wenting Li, Jiaming Liu, Heng Zhou, Yingxian Ma, Shen Ji, Yajun Cui
ABSTRACT

A simple, rapid, and effective method based on salting-out extraction and LC-MS/MS techniques was developed for the determination of 39 plant growth regulator (PGR) residues in two of the most common root and rhizome Chinese herbs, Codonopsis Radix (Dangshen) and Notoginseng Radix et Rhizoma (Sanqi). The extraction process was performed with acetonitrile-water (5:1) and citrate buffer extraction salt. The performance of the method was validated in accordance with the analytical quality control criteria of SANTE/11813/2017 guidelines. Analyte recoveries of 79.49-109.41% (Dangshen) and 80.17-102.81% (Sanqi) were achieved. The limit of quantifications (LOQs) were determined with the consideration of accuracy and precision. LOQs were lower than the lowest residue limits in EU pesticide regulation (10 µg/kg) for most PGRs. Moreover, the method was successfully applied in the analysis of 35 batches of Dangshen, and 60 batches of Sanqi products. The concentration of eleven PGRs were determined in analyzed samples.

MATERIALS
Product Number
Brand
Product Description

Supelco
Tebuconazole, PESTANAL®, analytical standard
Supelco
Mepiquat chloride, PESTANAL®, analytical standard
Supelco
Trinexapac-ethyl, PESTANAL®, analytical standard
Sigma-Aldrich
Indole-3-acetic acid sodium salt, BioReagent, suitable for plant cell culture, ≥98%
Supelco
α-tert-Butyl-β-(4-chlorobenzyl)-1H-1,2,4-triazole-1-ethanol, PESTANAL®, analytical standard
Sigma-Aldrich
Ancymidol, suitable for plant cell culture, BioReagent
Supelco
1-Naphthylacetamide, PESTANAL®, analytical standard
Supelco
α-tert-Butyl-β-(4-chlorobenzyl)-1H-1,2,4-triazole-1-ethanol, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland