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  • Advantages of pentafluorophenylpropyl stationary phase over conventional C18 stationary phase--application to analysis of triamcinolone acetonide.

Advantages of pentafluorophenylpropyl stationary phase over conventional C18 stationary phase--application to analysis of triamcinolone acetonide.

Talanta (2008-07-01)
Lucie Havlíková, Ludmila Matysová, Renata Hájková, Dalibor Satínský, Petr Solich
ABSTRACT

A pentafluorophenylpropyl (PFPP) stationary phase was for the first time tested for the simultaneous determination of triamcinolone acetonide, its degradation product triamcinolone and two preservatives, methylparaben, and propylparaben. A new simple isocratic reversed phase HPLC method with UV detection, using estradiol hemihydrate as an internal standard, has been developed and validated. Chromatography was performed on a Discovery HS F5 column (150 mm x 4.6 mm, 5 microm) using a binary mobile phase composed of acetonitrile and water 45:55 (v:v). The flow-rate was 0.6 mL/min, the column temperature 25 degrees C and the UV detection was accomplished at 240 nm. The chromatography results using PFPP stationary phase were compared with those obtained using conventional C18 columns.

MATERIALS
Product Number
Brand
Product Description

Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 5 cm × 4 mm
Supelco
Discovery® HS F5 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 2.1 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 10 cm × 4 mm
Supelco
Discovery® HS F5 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 2.1 mm, pkg of 1 kit
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 5 cm × 2.1 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
Supelco
Discovery® HS F5 Supelguard Cartridge, 3 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
Discovery® HS F5 Supelguard Cartridge, 3 μm particle size, L × I.D. 2 cm × 2.1 mm
Supelco
Discovery® HS F5 Supelguard Cartridge, 3 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 1 kit
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 2.1 mm
Supelco
Discovery® HS F5 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 21.2 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 2.1 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 10 cm × 4.6 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 5 cm × 4.6 mm
Supelco
Discovery® HS F5 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 1 kit
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 3 mm, pkg of 1 ea
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 3.3 cm × 2.1 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm
Supelco
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 10 cm × 2.1 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 2.1 mm
Supelco
Discovery® HS F5 Supelguard Cartridge, 3 μm particle size, L × I.D. 2 cm × 2.1 mm